Enzymes and Inhibitors
New England Biolabs, Inc. HiFi Taq DNA Ligase – 50 reactions
An optimized blend of a thermostable DNA Ligase and a proprietary additive, HiFi Taq DNA Ligase efficiently seals nicks in DNA with unmatched high fidelity. The formation of a phosphodiester bond between juxtaposed 5 phosphate and 3 hydroxyl termini of two adjacent oligonucleotides that are hybridized to a complementary target DNA is enhanced in the improved reaction buffer and mismatch ligation is dramatically reduced. The improved formulation allows higher resolution discrimination between ligation donors and acceptors, enabling precise detection of SNPs and other allele variants. HiFi Taq DNA Ligase is active at elevated temperatures (37-75C). Please note that HiFi Taq DNA Ligase is intended for use in molecular diagnostics applications that depend on high fidelity nick ligation. It is not a substitute for T4 DNA ligase and is not suitable for cloning applications or adapter ligation/NGS library prep.
Sigma Aldrich Fine Chemicals Biosciences Glutathione Reductase from baker's yeast (S. cerevisiae) ammonium sulfate suspension, 100-300 units/mg protein (biuret) | 9001-48-3 | MFCD00131196 | 100UN
Glutathione Reductase from baker's yeast (S. cerevisiae) ammonium sulfate suspension, 100-300 units/mg protein (biuret) | 9001-48-3 | MFCD00131196 | 100UN
Sigma Aldrich Fine Chemicals Biosciences ISOBUTYRYL COENZYME A LITHIUM
NC3840085 ISOBUTYRYL COENZYME A LITHIUM
AnaSpec ADAMTS-4/Aggrecanase FRET Substrate, FRET Assay, 1 mg
The ADAMTS-4/Aggrecanase FRET Substrate offers researchers a reliable tool for detecting ADAMTS-4 enzyme activity. With a quantity of 1 mg, this peptide substrate enables precise enzyme assays with FRET technology, distinguishing it from standard substrates. Its specific function in cartilage degradation pathways makes it vital for studies related to osteoarthritis and tissue remodeling. Easy to use and store, it supports ongoing research in enzyme substrates and inhibitors.
Sigma Aldrich Fine Chemicals Biosciences SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES
NC3915858 A-AMYLASE FROM HUMAN SALIVA
Ubpbio A FRET-based fluorogenic substrate of Papain-like protease (PLPro) from SARS viruses, including SARS-CoV-2. PLPro also has deubiquitinase activity. 5MG
Dabcyl–FRLKGGAPIKGV–Edans is an internally quenched fluorogenic substrate for the SARS-CoV or MERS-CoV family Papain-like protease (PLPro or PLP) that recognizes a LXGG consensus cleavage sites. Upon PLP-mediated cleavage between glycine and alanine, the resulting APIKGV-Edans fluorescence can be monitored at the excitation/emission wavelengths of 355/538 nm, respectively. In addition, PLP has deubiquitinating and deISG15ylation activities.
Abcam H-Met-AMC, Calpain substrate, 2G
MW 420.4 g/mol, Purity >99%. Calpain substrate. Achieve your results faster with highly validated, pure and trusted compounds.
The product is subject to the following: Abcam Restricted Use Statement
Medchemexpress LLC Ac-ANW-AMC | 2357123-49-8 | 99.4% | 10 MG
Ac-ANW-AMC is a fluorogenic peptide substrate used to measure immunoproteasome β5i activity in vitro. Proteolytic cleavage releases 7-amino-4-methylcoumarin (AMC), producing a fluorescent signal suitable for kinetic or end-point assays.
- releases AMC fluorophore upon proteolytic cleavage for fluorescence detection.
- reported excitation/emission: 380 nm/460 nm; assay notes recommend 345 nm/445 nm for β5i measurements.
- high purity (99.4%) for reliable biochemical assays.
- molecular weight 588.61 g/mol; formula C30H32N6O7.
- available in small quantities (for example, 10 mg) for research use.
- store at -20°C and protect from light; in solution, store at -80°C for long-term stability.
eMolecules 115144-35-9 | D-Luciferin potassium salt | Apollo Scientific | MFCD00044928 | 318.410 | C11H7KN2O3S2 | 0.000 | [K+].Oc1ccc2nc(sc2c1)C1=N[C@H](CS1)C([O-])=O | 100mg | 562533490
D-Luciferin potassium salt | Apollo Scientific | 115144-35-9 | MFCD00044928 | 318.410 | C11H7KN2O3S2 | 0.000 | [K+].Oc1ccc2nc(sc2c1)C1=N[C@H](CS1)C([O-])=O | 100mg | 562533490
Research Products International Corp 4-Methylumbelliferyl-B-L-Fucopyranoside, 100 Milligrams
Fluorescent substrate for β-L-Fucosidase.
- 4-Methylumbelliferone <50ppm
![Research Products International Corp 4-Methylumbelliferyl-B-D-glucopyranoside Monohydrate [Methylumbelliferyl-B-D-glucoside], 1 Gram](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-M65800-1.03.JPG-250.jpg)
Research Products International Corp 4-Methylumbelliferyl-B-D-glucopyranoside Monohydrate [Methylumbelliferyl-B-D-glucoside], 1 Gram
Fluorescent substrat for B-D-Glucosidase.
Enzo Life Sciences Ac-IETD-AFC (10 mg)
Caspase and granzyme substrate. Fluorogenic substrate for caspase-8/caspase-3 processing enzyme and granzyme B. Also a substrate for caspase-10. The IETD sequence is based on caspase-3 proenzyme cleavage site. Alternative Name: Caspase 8 substrate (fluorogenic), Granzyme B substrate (fluorogenic). Formula: C31H38F3N5O12. MW: 729.7. Purity: ≥98% (HPLC). Sequence Ac-Ile-Glu-Thr-Asp-AFC (AFC=7-Amino-4-trifluoromethylcoumarin). Appearance: White to off-white powder. Solubility: Soluble in DMSO. Long Term Storage: -20°C. Handling: Keep cool and dry.